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Layer‐by‐Layer Nano‐Encapsulation of Microbes: Controlled Cell Surface Modification and Investigation of Substrate Uptake in Bacteria |
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| Authors: | Bettina Franz Shantanu S Balkundi Christiane Dahl Yuri M Lvov Alexander Prange |
| Institution: | 1. Niederrhein University of Applied Sciences, Microbiology and Food Hygiene, Rheydter Strasse 277, 41065 Moenchengladbach, Germany;2. Institute for Microbiology & Biotechnology, University of Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany;3. Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Avenue, Ruston, LA 71272, USA;4. The J. Bennett Johnston Sr. Center for Advanced Microstructures & Devices (CAMD), Louisiana State University, 6980 Jefferson Highway, Baton Rouge, LA 70806, USA |
| Abstract: | LbL nano self‐assembly coating of A. vinosum with different polyelectrolyte combinations is presented as an example to investigate substrate uptake in bacteria. The effects of surface charge and the formation of a physical barrier provides new insights in the contact mechanisms between the cell surface and insoluble elemental sulfur. Furthermore, uptake of sulfide by encapsulated cells was investigated. Growth experiments of coated cells showed that surface charge did neither affect sulfide uptake nor the contact formation between the cells and solid sulfur. However, increasing layers slowed or inhibited the uptake of sulfide and elemental sulfur. This work demonstrates how defining surface properties of bacteria has potential for microbiological and biotechnological applications.  |
| Keywords: | Allochromatium vinosum layer‐by‐layer (LbL) self‐assembly surface modification |