1. European School of Molecular Medicine – SEMM, IFOM‐IEO campus, Via Adamello, 16 20139 Milan, Italy;2. Dipartimento di Chimica Organica e Industriale, Università di Milano, Via Venezian 21, I‐20133 Milan, Italy;3. CIMaINa, Dipartimento di Fisica, Università di Milano, Via Celoria 16, I‐20133 Milan, Italy;4. Fondazione Filarete, Viale Ortles 22/4, 20139 Milan, Italy;5. CIMaINa, Dipartimento di Scienze Molecolari Applicate ai Biosistemi, Università di Milano, Via Trentacoste 2, I‐20134 Milan, Italy
Abstract:
Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri‐cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell‐based arrays, stem cell therapy and cell‐based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub‐micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e‐beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti‐fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E‐beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.