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Characterization of Ultra‐Thin Temperature‐Responsive Polymer Layer and Its Polymer Thickness Dependency on Cell Attachment/Detachment Properties |
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| Authors: | Kazuhiro Fukumori Yoshikatsu Akiyama Yoshikazu Kumashiro Jun Kobayashi Masayuki Yamato Kiyotaka Sakai Teruo Okano |
| Affiliation: | 1. Department of Applied Chemistry, Waseda University, 3‐4‐1 Ohkubo, Shinjuku, Tokyo 169‐8555, Japan;2. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8‐1 Kawadacho, Shinjuku, Tokyo 162‐8666, Japan |
| Abstract: | Ultra thin poly(N‐isopropylacrylamide) (PIPAAm) modified glass coverslips (PIAPAm‐CS) using electron beam irradiation exhibited a clear relationship between the polymer thickness and thermal cell adhesion/detachment behavior. The polymer thickness dependency and the characteristic of ultra thin PIPAAm layer, has been illustrated in terms of the molecular motion of the modified PIPAAm chains. PIPAAm‐CSs surfaces with various area‐polymer densities and thicknesses were characterized by AFM and protein adsorption assay. The newly obtained results gave a further insight into the illustration. Finally, the future application of intelligent surfaces was discussed for fabricating tissue and organ.  |
| Keywords: | atomic force microscopy cell sheet engineering electron beam irradiation nanolayers poly(N‐isopropylacrylamide) |