Separation of α‐ from β‐arylalanines by nickel nitrilotriacetate chromatography |
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Authors: | Salinda Wijeratne Noelle A. Byrne Kevin D. Walker |
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Affiliation: | 1. Department of Chemistry, Michigan State University, East Lansing, MI, USA;2. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA |
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Abstract: | A method is described to separate α‐ from β‐arylalanines by ligand exchange chromatography on a nickel nitrilotriacetate agarose column with UV monitoring of the effluent. Separate mixtures containing an α‐ and β‐arylalanine pair (1 mg of each) were individually loaded onto the nickel resin pre‐equilibrated with the mobile phase at room temperature, and the amino acids were eluted from the column with a gradient from pH 12.0–8.0. The β‐arylalanines eluted first, followed by the α‐isomers. The four α/β‐amino acid pairs tested were well separated with baseline resolution. An aliquot of each fraction was chemically treated to derivatize the amino acids to their N‐acyl methyl ester analogs, and their identities were confirmed by GC/MS analysis. The sample recovery was quantitative (>98%), and the column matrix was very resilient, as demonstrated by consistent separation of the solutes after ~100 preparative cycles. |
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Keywords: | β ‐Amino acids Aminomutases Ni(II) chromatography |
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