毛细管区带电泳法同时测定饲料中西马特罗、盐酸克伦特罗和沙丁胺醇

建立了同时测定饲料中西马特罗、盐酸克伦特罗与沙丁胺醇的毛细管区带电泳-紫外检测方法。考察了实验参数对 分离和检测结果的影响。在最佳实验条件下,在60 mmol/L的柠檬酸-柠檬酸钠运行缓冲液(pH 6.29)中,上述3种物质在8 min内完全分离。西马特罗、盐酸克伦特罗和沙丁胺醇的线性响应范围为0.1~1.0 mg/L,最低检测限(以信噪比为3计)分 别为0.02,0.03和0.02 mg/L。所建立的方法直接用于饲料中西马特罗、盐酸克伦特罗和沙丁胺醇的测定,结果令人满意 。

Simultaneous Determination of Cimaterol, Clenbuterol and Salbutamol in Feeds by Capillary Zone

A method for simultaneous determination of cimaterol, clenbuterol and salbutamol in feeds was developed by capillary zone electrophoresis. The effects of the experimental conditions on the separation and determination of cimaterol, clenbuterol and salbutamol have been examined. Under the optimum conditions, all three compounds were completely separated in 8 min. The linear range of cimaterol, clenbuterol and salbutamol was 0.1-1.0 mg/L, and the detection limits (S/N=3) were 0.02, 0.03 and 0.02 mg/L, respectively. The developed method has been used for the determination of spiked feed samples. The recoveries of cimaterol, clenbuterol and salbutamol were 89%-103%, 86%-91% and 83%-87%, respectively. The relative standard deviations for cimaterol, clenbuterol and salbutamol were 3.8%-4.3%, 3.6%-5.0% and 4.0%-5.6%, respectively. The proposed method is a sensitive, fast and simple method for the determination of cimaterol, clenbuterol and salbutamol in feeds.