环氧基修饰周期性介孔有机氧化硅对漆酶的固定化作用

以三嵌段共聚物P123为模板剂, 在酸性条件下通过1,2-三乙氧基硅基乙烷(BTESE)和3-含氧缩水甘油基-丙基-三甲氧基硅烷(GPTMS)共水解缩聚合成环氧基修饰的周期性介孔氧化硅(PMOs), 以修饰后的PMOs为载体对漆酶进行固定化, 研究了环氧基修饰对固定化酶稳定性的影响. 通过XRD、TEM、固态NMR和N₂吸附等手段表征材料的修饰效果、孔结构以及漆酶的固定化. 结果表明, 修饰后的材料保持良好的孔结构, 环氧基的修饰有利于提高固定化酶的活力, 基于环氧基修饰PMOs的固定化酶具有较高稳定性.

Immobilization of Laccase on Epoxy Group-functionalized Periodic Mesoporous Organosilicas

Epoxy group-functionalized periodic mesoporous organosilicas(FPMOs) was synthesized by the co-condensation of 1,2-bis(triethoxysilyl)ethane(BTESE) and 3-glycidoxypropyltrimethoxylsilane(GPTMS) in the presence of triblock copolymer poly(ethylene glycol)-b-poly(propylene glycol)-b-poly(ethylene glycol)(P123) under acidic conditions and utilized as a support for laccase immobilization. The effect of epoxy group-functionalization on the immobilization of laccase and the stability of immobilized laccase were investigated in detail. The level of functionalization, the pore structure and the laccase immobilization were detected by means of solid-state NMR, N2 adsorption and UV-Vis, respectively. It is revealed that epoxy groups are successfully attached covalently to the pore wall of PMOs and the functionalized materials retain a desirable pore structure. Laccase immobilized on functionalized FPMOs exhibits a higher activity and stability than that adsorbed on pure PMOs.