Affinity separation of enzymes from mixtures containing suspended solids |
| |
Authors: | Bernard L Hirschbein George M Whitesides |
| |
Institution: | (1) Department of Chemistry, Massachusetts Institute of Technology, 02139 Cambridge, Massachusetts |
| |
Abstract: | Agarose beads containing immobilized enzymes or affinity ligands have been made magnetically responsive by adsorbing freshly
precipitated magnetite on their surface. These beads are used for affinity adsorption of proteins from complex mixtures containing
suspended solids. The magnetically responsive beads and the unwanted (diamagnetic) solids are then separated by magnetic filtration.
This magnetic adsorption scheme for direct affinity separation of enzymes from mixtures containing suspended solids is compared
with a similar, but nonmagnetic, scheme in which the affinity matrix is supported on fiberglass cloth. The enzyme is allowed
to adsorb in this matrix, and the matrix is simply removed physically from the suspension to achieve separation from the unwanted
solids. The two methods seem comparable in their ability to separate a desired enzymatic activity. The magnetic methods are
technically the more complex of the two, but are significantly the more rapid. The efficiency of separation of diamagnetic
and ferrimagnetic solids in these biological systems by high gradient magnetic filtration is good. |
| |
Keywords: | Affinity separation of enzymes via magnetic techniques separation of enzymes by magnetic affinity techniques enzymes separation by magnetic affinity techniques solids affinity separation of enzymes from mixtures of suspended magnetic separation of enzymes from suspended solids |
本文献已被 SpringerLink 等数据库收录! |