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Microbial and enzymatic hydrolysis of tannic acid: influence of substrate chemical quality
Authors:Mónica Lizeth Chávez-González  Juan Carlos Contreras-Esquivel  Lilia Arely Prado-Barragán  Raúl Rodríguez  Antonio Francisco Aguilera-Carbo  Luis Víctor Rodríguez  Cristóbal Noé Aguilar
Affiliation:1.Department of Food Science and Technology, School of Chemistry,Autonomous University of Coahuila,Saltillo, Coahuila,Mexico;2.Department of Biotechnology, Division of Health and Biological Sciences,Metropolitan Autonomous University,Mexico City,Mexico;3.Department of Food Science and Technology, Division of Animal Sciences,Antonio Narro Agrararian Autonomous University,Saltillo, Coahuila,Mexico
Abstract:Tannic acid is commonly employed as the main component in culture media for the selection of tannase-producing strains. In biotechnological processes it is the favorite substrate used to induce the tannase enzyme in both solid and submerged culture for microbial and/or enzymatic production of gallic acid. However, the results found in literature are inconsistent notwithstanding the strict control of all parameters that rule the bioprocesses. The present work, for the first time, reveals the importance of differences in the quality and chemical profile of tannic acid from different suppliers and their influence on the fungal and enzymatic hydrolytic pattern obtained when it is used as a substrate. A degree of hydrolysis between 64.7 % and 100 % has been determined in different tannic acid samples. The specific growth rate of 0.712 h?1, 0.792 h?1, 0.477 h?1, 0.536 h?1 for Jalmek®, Faga Lab®, Division Food®, and Riedel de Häen®, respectively, was obtained at the concentration of 80 g L?1 of each of the tannic acids.
Keywords:
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