Whole Cells in Enantioselective Reduction of tert-Butyl Acetoacetate |
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| Authors: | Aline de Souza Ramos Raquel de Oliveira Lopes Rodrigo Octavio Mendonça Alves de Souza |
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| Institution: | 1. Farmanguinhos, Funda??o Oswaldo Cruz , Rio de Janeiro , Brazil;2. Biocatalysis and Organic Synthesis Group, Instituto de Química, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil |
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| Abstract: | The β-ketoester tert-butyl acetoacetate was enantioselectively reduced to tert-butyl (S)-3-hydroxybutanoate by seven microorganism strains. The best result using free cells was obtained with the yeast R. rubra, which furnished 97.6% ee and higher than 99% of conversion within 24 h. After immobilization in calcium alginate spheres, R. rubra furnished 96% ee and higher than 99% ee within 24 h, even if substrate concentration was 58 mM. Immobilized cells were reused three times without loss of enantioselectivity. |
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| Keywords: | Calcium alginate chiral reduction immobilization β-ketoester R rubra |
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