Artificial Enzyme Mimics for Catalysis and Double Natural Enzyme Co-immobilization

This work presents a new chemiluminescent (CL) probe array assay. The new type CL probe array is based on enzyme mimics of Co₃O₄–SiO₂ mesoporous nanocomposite material, which not only have an excellent catalytic effect on the luminol–H₂O₂ CL reaction in an alkaline medium but also can be used for the immobilization of enzymes. The linear range of the lactose concentration is 3.0?×?10^?7 to 1.0?×?10^?5 g mL^?1 and the detection limit is 6.9?×?10^?8 g mL^?1. β-Galactosidase and glucose oxidase were selected as a model for enzyme assays to demonstrate the applicability of Co₃O₄–SiO₂ mesoporous nanocomposite material in multienzyme immobilization. The novel bifunctional CL probe array has been successfully applied to the determination of lactose in milk.