Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS |
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Authors: | Macháň Radek Hof Martin Chernovets Tatsiana Zhmak Maxim N Ovchinnikova Tatiana V Sýkora Jan |
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Institution: | (1) J. Heyrovsk? Institute of Physical Chemistry v.v.i, Academy of Sciences of the Czech Republic, Dolejškova 2155/3, 182 23 Prague 8, Czech Republic;(2) Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str. 16/10, 117997 Moscow, Russia; |
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Abstract: | Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported
lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are
used to detect changes in lipid and peptide diffusion upon addition of unlabelled arenicin-1 to SLBs. Arenicin-1 decreases
lipid mobility in negatively charged SLBs. According to diffusion law analysis, microdomains of significantly lower lipid
mobility are formed. The analysis of peptide FCS data confirms the presence of microdomains for anionic SLBs. No indications
of microdomain formation are detected in SLBs composed purely of zwitterionic lipids. Additionally, our FCS results imply
that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions. |
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