Solid/liquid interfacial tension as a tool to study stability of lysozyme on adsorption to solid surfaces |
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| Institution: | 1. Centre for Biotechnology, Anna University, Chennai 600 025, India;2. Chemical Laboratory, CLRI, Sardar Patel Road, Adyar, Chennai 600020, India;1. Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Av Joan XXIII s/n, 08028 Barcelona, Spain;2. Department of Chemical and Biomolecular Nanotechnology, IQAC-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain;3. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, Barcelona, Spain;1. Key Laboratory of Soft Chemistry and Functional Materials, Ministry of Education, Nanjing University of Science and Technology, Nanjing 210014, China;2. Collaborative Innovation Center for Biomedical Functional Materials, Nanjing Normal University, Nanjing 210097, China;1. Max Planck Institute of Colloids and Interfaces, D-14424, Golm, Germany;2. Institute of Physical Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;3. Institute of Colloid Chemistry and Chemistry of Water, Kyiv (Kiev), Ukraine;4. Medical University Donetsk, Donetsk, Ukraine;1. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China;2. College of Physics, Optoelectronics and Energy of Soochow University, Suzhou 215006, PR China |
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| Abstract: | This work proposes the use of solid/liquid interfacial tension to study the stability of adsorbed lysozyme films on a solid surface using the contact angle of a liquid at the three phase contact line, in the presence of a denaturant, urea.Results suggest a direct correlation between this method with a standard technique like the fluorescence emission spectra and is measured with the same observable error as in the spectral methods. Further the technique provides a simple and direct handle to evaluate the homogeneity and degree of polarity of protein films on solid surfaces. |
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