纳米金探针检测Hg 2+离子 |
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引用本文: | 鲍军方a 何 鹏a 于锡娟,a 姜世丽b 颜清云a.纳米金探针检测Hg 2+离子[J].化学学报,2009,67(18):2139-2143. |
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作者姓名: | 鲍军方a 何 鹏a 于锡娟 a 姜世丽b 颜清云a |
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作者单位: | (a青岛科技大学化学与分子工程学院 青岛 266042) ;(b山东省莱阳卫生学校内科教研室 莱阳 265200) ; |
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摘 要: | 利用Hg2+的核酸适体修饰纳米金形成探针建立了一种定量检测Hg2+离子的方法. Hg2+适体吸附在纳米金表面, 使纳米金的稳定性增强, 抑制氯化钠对纳米金的团聚作用. 溶液中有Hg2+离子存在时, 由于适体与纳米金的吸附作用小于适体与Hg2+离子的亲和作用, 纳米金失去适体保护在氯化钠作用下发生团聚. 溶液颜色由红变蓝, 紫外-可见光谱最大吸收峰由520 nm红移至620 nm. 在优化条件下, 吸光度的比值(A620/A520)与Hg2+离子浓度在5.0×10-9~7.2×10-7 mol&;#8226;L-1范围内呈线性关系, 检测限可达3.3×10-10 mol&;#8226;L-1. 研究了K+, Ca2+等常见离子的干扰, 结果表明该方法具有良好的选择性.
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关 键 词: | 纳米金 核酸适体 Hg2+ 紫外-可见光谱 |
收稿时间: | 2008-9-16 |
修稿时间: | 2009-2-20 |
Detection of Hg 2+ Using Gold Nanoparticle Probes |
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Institution: | ( College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042)
( Department of Internal Medicine, Laiyang Medical School, Laiyang 265200) |
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Abstract: | A method was developed for Hg2+ quantitative determination using the Hg2+ aptamer to modify gold nanoparticles (AuNP). The aptamer would adsorb onto the AuNP and help to enhance the AuNP stability against salt-induced aggregation. But this attraction was weaker than the affinity between the target (Hg2+) and the aptamer. So if the proper Hg2+ and aptamer were put into the AuNP solution, addition of salt resulted in aggregation of AuNP that led to red-to-blue color change. And the maximum absorption wavelength would change from 520 to 620 nm. Before detecting Hg2+, the concentrations of NaCl and aptamer were optimized, and the appropriate pH was chosen. Under optimal conditions, the absorption ratio A620/A520 was proportional to the concentration of Hg2+ in the range of 5.0×10-9 to 7.2×10-7 mol&;#8226;L-1 and the detection limit was 3.3×10-10 mol&;#8226;L-1. Interferences from selected inorganic ions were investigated and the proposed method was simple and sensitive. |
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Keywords: | gold nanoparticle aptamer Hg2+ UV-Vis spectra |
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