| 凝胶色谱-光散射联用法对壳聚糖及其产品的分子质量及分布的测量 |
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| 引用本文： | 任玲玲,祁欣,莫洪波. 凝胶色谱-光散射联用法对壳聚糖及其产品的分子质量及分布的测量[J]. 分析测试学报, 2010, 29(1). DOI: 10.3969/j.issn.1004-4957.2010.01.008 |
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| 作者姓名： | 任玲玲 祁欣 莫洪波 |
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| 作者单位： | 中国计量科学研究院,北京,100013;重庆市计量质量检测研究院,重庆,400020 |
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| 基金项目： | 中国计量科学研究院博士专项基金 |
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| 摘 要： | 采用国家基准物质氯化钠和甲苯校准了示差折光仪和增强光系统的仪器常数,用超纯水重量法校准了激光光散射仪到示差检测器之间的延时体积,用葡聚糖标准物质对各角度的光强进行归一化处理,研究了凝胶色谱-激光光散射联用方法中设备的校准和溯源。上述校准途径使仪器溯源到国际SI单位,从而确保了测量结果准确可靠。采用激光光散射仪测得壳聚糖及其产品术后防粘连隔离膜的光折射增量dn/dc值分别为0.118、0.092 mL/g,凝胶色谱-激光光散射联用方法测得壳聚糖及其产品术后防粘连隔离膜的重均分子质量分别为8.897×104、1.168×105g/mol;分子质量分布Mw/Mn分别为1.148±0.026和3.132±0.377。
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| 关 键 词： | 壳聚糖 术后防粘连隔离膜 分子质量 凝胶色谱 激光光散射 |
Accurate Measurement of Molecular Mass and Molecular Mass Distribution of Chitosan and Its Products by GPC-Laser Light Scatter Method |
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| REN Ling-ling,QI Xin,MO Hong-bo. Accurate Measurement of Molecular Mass and Molecular Mass Distribution of Chitosan and Its Products by GPC-Laser Light Scatter Method[J]. Journal of Instrumental Analysis, 2010, 29(1). DOI: 10.3969/j.issn.1004-4957.2010.01.008 |
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| Authors: | REN Ling-ling QI Xin MO Hong-bo |
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| Affiliation: | REN Ling-ling1,QI Xin1,MO Hong-bo2(1.National Institute of Metrology,Beijing 100013,China,2.Chongqing Institute of Metrology , Quality Inspection,Chongqing 400020,China) |
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| Abstract: | The differential refraction detector and enhanced optical system of laser scatter instrument were calibrated by the primary standard NaCl and the certified reference material toluene,respectively.The delay volume between enhanced optical system and differential refraction detector was also calibrated by the certified reference material polystyrene.The light strength of different angles was normalized by the certified reference material of glucan.All of these processes ensured the measurement results to be a... |
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| Keywords: | chitosan the separate membrane to prevent adhesions molecular mass gel permeation chromatography laser light scatter |
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