| IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX |
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| 引用本文： | 王玲治,江英彦,张长德,黄德琇. IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX[J]. 高分子科学, 1990, 0(2): 115-120 |
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| 作者姓名： | 王玲治 江英彦 张长德 黄德琇 |
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| 作者单位： | Institute of Chemistry，Academia Sinica，Beijing，Institute of Chemistry，Academia Sinica，Beijing，Xi'an Jiaotong University，Xi'an，China，Xi'an Jiaotong University，Xi'an，China |
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| 摘 要： | This study is concerned with chitosan-polyacrylic acid complex as a carrier to immobilize glucose oxidase (GOD)and cellulase. The optimum emperature of the immobilized GOD (IG) was determined to be 60℃which is higher than that of the native GOD about 40℃. The optimum temperature of the immobilized cellulase (IC) was determined to be about 30℃higher than that of native cellulase. Both of the optimum pH of IG and IC shifted one pH unit to acid. Immobilized enzyme may be used in more wide pH range. Their storage life are much longer compared with their native states. Both of them can be reused at least 12 times.
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| 收稿时间： | 1988-09-16 |
IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX |
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| WANG Lingzhi,JIANG Yingyan,ZHANG Changde,HUANG Dexiu. IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX[J]. Chinese Journal of Polymer Science, 1990, 0(2): 115-120 |
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| Authors: | WANG Lingzhi JIANG Yingyan ZHANG Changde HUANG Dexiu |
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| Affiliation: | Institute of Chemistry; Academia Sinica; Beijing; Xi'an Jiaotong University; Xi'an; China |
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| Abstract: | This study is concerned with chitosan-polyacrylic acid complex as a carrier to immobilize glucose oxidase (GOD)and cellulase. The optimum emperature of the immobilized GOD (IG) was determined to be 60℃ which is higher than that of the native GOD about 40℃. The optimum temperature of the immobilized cellulase (IC) was determined to be about 30℃ higher than that of native cellulase. Both of the optimum pH of IG and IC shifted one pH unit to acid. Immobilized enzyme may be used in more wide pH range. Their storage life are much longer compared with their native states.Both of them can be reused at least 12 times. |
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| Keywords: | Immobilized enzyme Cellulase Glucose oxidase Polymer complex |
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