Immobilization of ABTS – laccase system in silicate based electrode for biolectrocatalytic reduction of dioxygen |
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| Authors: | Wojciech Nogala Ewa Rozniecka Izabella Zawisza Jerzy Rogalski Marcin Opallo |
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| Institution: | aInstitute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland;bDepartment of Chemistry, University of Oldenburg, Carl von Ossietzky Strasse 9/11, 26-129 Oldenburg, Germany;cDepartment of Biochemistry, Maria Curie Sklodowska University, Lublin, Poland |
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| Abstract: | Voltametrically stable 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS2−) modified electrode was obtained by sol–gel processing of methyltrimethoxysilane based sol with dissolved ABTS2− together with dispersed graphite particles. Next, extracellular laccase from Cerrena unicolor was encapsulated within a thin hydrophilic tetramethoxysilane film on the top the electrode. The obtained ABTS2− modified carbon ceramic electrode exhibits stable voltammetry corresponding to the surface confined oxidation reduction process. The biocatalytic activity of this electrode is similar to that observed when ABTS2− is present in solution. |
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| Keywords: | Laccase ABTS Dioxygen reduction Sol– gel Carbon ceramic electrode Bioelectrocatalysis |
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