| 柱前衍生高效液相色谱法测定NTCA的光学纯度 |
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| 引用本文： | 都婷婷,黎利军,宋航,张义文,王欣,罗佳.柱前衍生高效液相色谱法测定NTCA的光学纯度[J].分析试验室,2009,28(6). |
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| 作者姓名： | 都婷婷 黎利军 宋航 张义文 王欣 罗佳 |
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| 作者单位： | 四川大学制药与生物工程系,成都,610065 |
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| 摘 要： | 用1-萘胺对乙酰四氢噻唑-2-硫酮-4-羧酸进行柱前衍生,产生非对映体衍生物.以正己烷和乙醇或异丙醇为流动相,选择Chiralcel OD-H色谱柱上对NTCA衍生物进行了拆分,考察了流动相组成和柱温对其衍生物分离的影响,获得较好的分析条件,分离因子达到1.3.该结果进一步与旋光仪法相比较,相对偏差不超过1.9%.
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| 关 键 词： | 乙酰四氢噻唑-2-硫酮-4-羧酸 高效液相色谱 柱前衍生化 光学纯度 |
Determination of enantiomeric purity of(R)-N-acetylthiazolidine-2-thione-4-carboxylic acid by high performance liquid chromatography with pre-column derivatization |
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| DU Ting-ting,LI Li-jun,SONG Hang,ZHANG Yi-wen,WANG Xin,LUO Jia.Determination of enantiomeric purity of(R)-N-acetylthiazolidine-2-thione-4-carboxylic acid by high performance liquid chromatography with pre-column derivatization[J].Chinese Journal of Analysis Laboratory,2009,28(6). |
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| Authors: | DU Ting-ting LI Li-jun SONG Hang ZHANG Yi-wen WANG Xin LUO Jia |
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| Institution: | Department of Pharmaceutical and Biological Engineering;Sichuan University;Chengdu 610065 |
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| Abstract: | Normal-phase chromatographic model was employed for enantionmeric separation of(R)-N-acetylthiazolidine-2-thione-4-carboxylic acid derivatives on the Chiralcel OD-H column in hexane/isopropyl alcohol mobile phase.The effects of chromatographic conditions such as mobile phases and temterature to chiral recognition of the N-acetylthiazolidine-2-thione-4-carboxylic acid detrivatives have been investigated.The optimal conditions of analysis were obtained and the separation factor was more than 1.3.Furthermore,t... |
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