Effective bioelectrocatalysis of bilirubin oxidase on electrochemically reduced graphene oxide |
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| Institution: | 1. Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, 28871, Alcalá de Henares, Madrid, Spain;2. Instituto de Investigación Química “Andrés M. del Río”, Universidad de Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, 28871, Alcalá de Henares, Madrid, Spain;3. Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, 28871, Alcalá de Henares, Madrid, Spain;4. Networking Research Center for Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Spain;5. Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Spain;1. Department of Surgery, University of Zagreb, School of Medicine, Clinical Hospital Center Zagreb – “Rebro”, Zagreb, Croatia;2. Department of Radiology, University of Zagreb, School of Medicine, Clinical Hospital Center Zagreb – “Rebro”, Zagreb, Croatia;3. Department of Transfusion Medicine and Transplantation Biology, University of Zagreb, School of Medicine, Clinical Hospital Center Zagreb – “Rebro”, Zagreb, Croatia;1. Faculty of Chemistry, Razi University, Kermanshah, Iran;2. Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran;3. Department of Materials Science, University of Milano-Bicocca, Via Cozzi 55, 20125 Milan, Italy;4. Department of Chemistry and Biomolecular Sciences, Clarkson University, Potsdam, NY 13699-5810, USA;5. Research Center of Oils and Fats, Kermanshah University of Medical Sciences, Kermanshah, Iran;6. Department of Chemistry, University of Bari A. Moro, 70125 Bari, Italy;1. Amrita Biosensor Research Lab, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, 641112, India;2. Department of Sciences, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, 641112, India;3. Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam 690525, India |
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| Abstract: | Graphene oxide (GO) was applied for construction of an effective biocathode based on bilirubin oxidase (BOD). Separation of small-sized GO sheets together with the BOD immobilisation protocol has detrimental effects on the bioelectrocatalytic reduction of oxygen. When BOD was deposited on electrochemically reduced GO (ErGO) only a negligible current density j = 2.6 μA cm? 2 was observed. Current density dramatically increased to a value of 46 μA cm? 2 once BOD was in-situ mixed with as-received GO directly on a glassy carbon electrode (GCE) with subsequent electrochemical reduction of the BOD/GO composite. When this protocol was tested with small-sized GO flakes separated simply using centrifugation, the fabricated biocathode exhibited j = 120 μA cm? 2. A current density further increased to j = 280 μA cm? 2 when BOD and purified GO were incubated ex-situ for 4 h, followed by the BOD/GO composite collection by centrifugation, its deposition on the GCE and electrochemical reduction. Moreover, oxygen reduction current increased steeply with a steady-state current density achieved at high potential (≈ 500 mV), close to the onset potential of oxygen reduction (≈ 580 mV). |
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