Preparation of anhydrochymotrypsin diol silica as selective adsorbent for affinity chromatography of peptides with aromatic amino acids at C-termini |
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Authors: | T Ohta K Ishimura S Takitani |
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Institution: | (1) Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya-Funagawara-Machi, Shinjuku-Ku, 162 Tokyo, Japan |
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Abstract: | Summary Anhydrochymotrypsin (AHC), a catalytically inert derivative of chymotrypsin in which the serine-residue active site has been converted chemically to a dehydroalanine residue, was immobilized on diol silica by activation with trifluoroethanesulfonyl chloride. A AHC-diol-silica column was used for high-performance affinity chromatographic separation of peptides with aromatic amino acids at their C-termini from other peptides. Faster separations were achieved. |
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Keywords: | Affinity chromatography Anhydrochymotrypsin Peptides with aromatic amino acids at C-termini Diol silica |
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