1.Key Laboratory of Luminescent and Real-Time Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People’s Republic of China ;
Abstract:
An ultrasensitive electrochemical glucose biosensor has been developed by depositing C60-fullerene functionalized with tetraoctylammonium bromide (C60-TOAB+) on the surface of a glassy carbon electrode (GCE). The glucose-binding protein concanavalin A (Con A) was then linked to the surface. Binding of glucose by Con A affects the electroactivity of the reversible redox couple C60/C60−, and this finding forms the basis for a quantitative glucose assay over the 10 to 10 mM concentration range and with a lower detection limit of 3.3 nM (at an S/N ratio of 3). The sensitivity of this sensor allowed glucose to be determined in saliva. This biosensor possesses excellent selectivity, outstanding reproducibility and good long-term stability.
An ultrasensitive electrochemical glucose biosensor has been developed by depositing C60-fullerene functionalized with tetraoctylammonium bromide (C60-TOAB+) on the surface of a glassy carbon electrode (GCE). The glucose-binding protein concanavalin A (Con A) was then linked to the surface. Binding of glucose by Con A affects the electroactivity of the reversible redox couple C60/C60−, and this finding forms the basis for a quantitative glucose assay over the 10 to 10 mM concentration range and with a lower detection limit of 3.3 nM (at an S/N ratio of 3). The sensitivity of this sensor allowed glucose to be determined in saliva.