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Direct electrochemistry of glucose oxidase and sensing glucose using a screen-printed carbon electrode modified with graphite nanosheets and zinc oxide nanoparticles |
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| Authors: | Karuppiah Chelladurai Palanisamy Selvakumar Chen Shen-Ming Veeramani Vediyappan Periakaruppan Prakash |
| Affiliation: | 1.Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan, Republic of China ;2.Post Graduate and Research Department of Chemistry, Thiagarajar College, Madurai, 625009, Tamil Nadu, India ; |
| Abstract: | We have studied the direct electrochemistry of glucose oxidase (GOx) immobilized on electrochemically fabricated graphite nanosheets (GNs) and zinc oxide nanoparticles (ZnO) that were deposited on a screen printed carbon electrode (SPCE). The GNs/ZnO composite was characterized by using scanning electron microscopy and elemental analysis. The GOx immobilized on the modified electrode shows a well-defined redox couple at a formal potential of −0.4 V. The enhanced direct electrochemistry of GOx (compared to electrodes without ZnO or without GNs) indicates a fast electron transfer at this kind of electrode, with a heterogeneous electron transfer rate constant (Ks) of 3.75 s−1. The fast electron transfer is attributed to the high conductivity and large edge plane defects of GNs and good conductivity of ZnO-NPs. The modified electrode displays a linear response to glucose in concentrations from 0.3 to 4.5 mM, and the sensitivity is 30.07 μA mM−1 cm−2. The sensor exhibits a high selectivity, good repeatability and reproducibility, and long term stability.  Graphical representation for the fabrication of GNs/ZnO composite modified SPCE and the immobilization of GOx |
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