Determination and pharmacokinetics of periplocin in rat plasma by LC‐MS |
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Authors: | Lixin Yi Kaishun Bi Xinyang Chen Qiufeng Zhang Shuang Che Wentao Liu Dan Lu Xiaohui Chen |
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Affiliation: | School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning 110016, People's Republic of China |
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Abstract: | A simple and sensitive LC‐MS method for the determination of periplocin in rat plasma was developed and validated. The chromatographic separation was carried out using a reverse‐phase Kromasil C18 column(150 × 4.6 mm, i.d., 5 µm) with a mobile phase composed of methanol–water (76:24, v/v). The flow rate of mobile phase was 0.8 mL/min. The calibration curve was linear within the concentration range 1–1000 ng/mL. The intra‐ and inter‐day precisions across three validation days over the entire concentration range was lower than 9.2% in terms of relative standard deviation. Accuracy determined at three quality control concentrations ranged from ?2.0 to 6.0% in terms of relative error. The validated method was applied to the pharmacokinetic study of periplocin in rat plasma after intravenous and intramuscular administration. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | periplocin rat plasma pharmacokinetics LC‐MS |
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