Determination of serum cholestanol by semi‐micro high‐performance liquid chromatography with electrochemical detection |
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| Authors: | Kazuhiro Hojo Hideki Hakamata Aya Takahashi Yu‐Ya Hosokawa Fumiyo Kusu |
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| Affiliation: | 1. Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi 1432‐1, Hachioji, Tokyo 192‐0392, Japan;2. Pharmaceutical Analysis I, Pharmaceutical Research and Technology Laboratories, Astellas Pharma Inc., 180 Ozumi, Yaizu, Shizuoka 425‐0072, Japan |
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| Abstract: | A simple and sensitive method that dose not require derivatization for determining cholestanol has been developed using HPLC with electrochemical detection (HPLC‐ECD). The current peak height was linearly related to the amount of cholestanol injected, ranging from 1 to 200 μM (r = 0.999). The detection limit (S/N = 3) of cholestanol was 0.23 μM (1.2 pmol). Total cholestanol in control human and mouse serum was determined by the present method with a recovery rate of more than 90% and an RSD (n = 5) of less than 7.3%. Further, this method was successfully applied to monitor experimental hypercholestanolemia in mice fed a high‐cholestanol diet, an animal model of cerebrotendinous xanthomatosis (CTX). In conclusion, we found this method to be both simple and useful for the determination of cholestanol in serum, helping in the diagnosis of CTX. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | cholestanol cholesterol HPLC cerebrotendinous xanthomatosis electrochemical detection |
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