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Chiral separation of the β2‐sympathomimetic fenoterol by HPLC and capillary zone electrophoresis for pharmacokinetic studies |
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| Authors: | Dirk Wesenberg Corinna Bleuel Gerd‐Joachim Krauss Martin G Schmid Michael Weiss Gerald Gübitz |
| Institution: | 1. Faculty of Biosciences, Institute of Biochemistry and Biotechnology, Martin Luther University Halle‐Wittenberg, Kurt‐Mothes‐Str. 3, D‐06120 Halle (Saale), Germany;2. Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Karl‐Franzens University, Universit?tsplatz 1, A‐8010 Graz, Austria;3. Faculty of Medicine, Institute of Pharmacology, Martin Luther University, Magdeburger Str. 4, D‐06120 Halle (Saale), Germany |
| Abstract: | The development of methods for the separation of the enantiomers of fenoterol by chiral HPLC and capillary zone electrophoresis (CZE) is described. For the HPLC separation precolumn fluorescence derivatization with naphthyl isocyanate was applied. The resulting urea derivatives were resolved on a cellulose tris(3,5‐dimethylphenylcarbamate)‐coated silica gel column employing a column switching procedure. Detection was carried out fluorimetrically with a detection limit in the low ng/mL range. The method was adapted to the determination of fenoterol enantiomers in rat heart perfusates using liquid–liquid extraction. As an alternative a CE method was used for the direct separation of fenoterol enantiomers comparing different cyclodextrin derivatives as chiral selectors. Copyright © 2010 John Wiley & Sons, Ltd. |
| Keywords: | fenoterol precolumn derivatization 1‐naphthyl isocyanate S‐(+)‐1‐(1‐naphthyl)‐ethylisocyanate enantioselective HPLC Chiracel OD‐RH chiral CE cyclodextrins column switching |