Identification of seven metabolites of oxyresveratrol in rat urine and bile using liquid chromatography/tandem mass spectrometry |
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| Authors: | Huilian Huang Guangtong Chen Zhiqiang Lu Jinqiang Zhang De‐An Guo |
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| Institution: | 1. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 199 Guo Shoujing Road, Zhangjiang, Shanghai 201203, People's Republic of China;2. Key Laboratory of Modern Preparation of TCM, Jiangxi University Of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, People's Republic of China |
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| Abstract: | Oxyresveratrol (trans‐2,4,3′,5′‐tetrahydroxystilbene) is a major compound isolated from Smilax china, a Chinese herbal medicine. The rat urine and bile samples were pretreated by solid‐phase extraction method after oral administration at a dose of 100 mg/kg of oxyresveratrol. Seven metabolites were identified by LC‐MS/MS method with electrospray ionization in negative ion mode. The results indicated that main metabolites of oxyresveratrol were monoglucuronided and monosulfated oxyresveratrol. Based on the results, the metabolic pathway of oxyresveratrol in rat urine and bile was proposed. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | oxyresveratrol metabolite LC‐MS/MS SPE |
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