High‐performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats |
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| Authors: | Rebecca M Bertram Jody K Takemoto Connie M Remsberg Karina R Vega‐Villa Shyam Sablani Neal M Davies |
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| Institution: | 1. College of Pharmacy Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program, Washington State University, Pullman, Washington, 99164–6534, USA;2. College of Agricultural, Human and Natural Resource Sciences, Biological Systems Engineering, Washington State University, Pullman, Washington, 99164–6534, USA |
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| Abstract: | A high‐performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna® C18 column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5–100.0 μg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069–18.4% (RSD%), and within 20% at the limit of quantitation (0.5 μg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre‐clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | oxyresveratrol stilbene HPLC nutraceutical pharmacokinetics |
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