HPLC of basic drugs using non‐aqueous ionic eluents: evaluation of a 3 μm strong cation‐exchange material |
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| Authors: | Phillip E Morgan Victoria Manwaring Robert J Flanagan |
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| Institution: | 1. Toxicology Unit, Department of Clinical Biochemistry, King's College Hospital NHS Foundation Trust, Denmark Hill, London SE5 9RS, UK;2. School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK |
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| Abstract: | HPLC columns packed with 3 μm particle size HPLC Technology Techsphere SCX (propylsulfonic acid‐modified) silica offer considerable advantages over 5 μm SCX packings in the analysis of basic drugs using 100% methanol eluents containing an ionic modifier such as ammonium perchlorate. The basic drugs studied included clozapine and norclozapine, olanzapine, quinine and quinidine, and amitriptyline, nortriptyline, imipramine and desipramine. The 3 μm column was not only more efficient for a given column length compared with 5 μm materials, but also elution times were less, a phenomenon observed in reversed‐phase systems. The high efficiencies and excellent peak shapes obtained with the 3 μm SCX‐modified packing together with the relatively low back‐pressures attained show that such materials deserve serious consideration by laboratories involved in the analysis of basic drugs. Manufacturers should offer such packings as a matter of routine. Alternative ionic modifiers such as ammonium acetate are available for use with mass spectrometric detection if required. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | basic drugs non‐aqueous eluents 3 micron SCX |
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