A liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of escin Ia and escin Ib in human plasma: application to a pharmacokinetic study after intravenous administration |
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Authors: | Lidong Liu Xiujun Wu Dan Wu Yingwu Wang Pengfei Li Yantong Sun Yan Yang Jingkai Gu Yimin Cui |
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Institution: | 1. Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130021, People's Republic of China;2. Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110041, People's Republic of China;3. Department of Pharmacy, Peking University First Hospital, Beijing, 100034, People's Republic of China |
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Abstract: | A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for simultaneous quantification of escin Ia and escin Ib in human plasma. After a solid‐phase extraction (SPE), the analytes were separated on a Zorbax Extend C18 column by isocratic elution with a mobile phase of methanol–acetonitrile–10 mm ammonium acetate (27:27:46, v/v/v) at a flow rate of 1.0 mL/min and analyzed by mass spectrometry in the positive ion multiple reaction monitoring mode. The precursor to product ion transitions of m/z 1131.8 → 807.6 was used to quantify escin Ia and escin Ib. Good linearity was achieved over a wide range of 2.00–900 ng/mL for escin Ia and 1.50–662 ng/mL for escin Ib. The intra‐ and inter‐day precisions (as relative standard deviation) were less than 11% for each QC level of escin Ia and escin Ib. The accuracies (as relative error) were within ±5.27% for escin Ia and within ±4.07% for escin Ib. The method was successfully employed in a pharmacokinetic study after a single intravenous infusion administration of sodium aescinate injection containing 10 mg escin to each of the 10 healthy volunteers. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | escin Ia escin Ib LC‐MS/MS pharmacokinetics |
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