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Interdisciplinary study for the evaluation of biochemical alterations on mussel <Emphasis Type="Italic">Mytilus galloprovincialis</Emphasis> exposed to a tributyltin-polluted area |
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| Authors: | Emanuele Magi Camilla Liscio Erika Pistarino Barbara Santamaria Marina Di Carro Micaela Tiso Andrea Scaloni Giovanni Renzone M Elisabetta Cosulich |
| Institution: | (1) Department of Chemistry and Industrial Chemistry, University of Genoa, 16146 Genoa, Italy;(2) Proteomic and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy;(3) Department of Chemistry and Industrial Chemistry, Via Dodecaneso 31, 16146 Genoa, Italy |
| Abstract: | An interdisciplinary approach was employed to monitor the concentration and the effects of butyltin compounds in mussels (Mytilus galloprovincialis). Tissues from animals exposed to a marine area (Vado Ligure harbour) with a high concentration of tributyltin (TBT) were analysed and compared with control samples. TBT concentrations were measured by gas chromatography–mass spectrometry and the protein pattern in gill tissues was studied by proteomic analysis. Several proteomic signatures associated with contaminant exposure were observed; spots that were significantly increased in all contaminated samples were identified by mass spectrometry as fragments of β-tubulin. The degradation of β-tubulin was then confirmed by western blot analysis with specific anti-β-tubulin antibody. The effects observed on mussel gills after exposure in the TBT-polluted area are discussed. |
| Keywords: | Tributyltin Mytilus galloprovincialis Proteomic analysis Mass spectrometry Immunodetection |
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