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Oxidative stress and defence mechanisms of the freshwater cladoceran Daphnia longispina exposed to UV radiation
Authors:Vega M P  Pizarro R A
Affiliation:Comisión Nacional de Energía Atómica, Dpto. Radiobiología, Buenos Aires, Argentina. pamavega@cnea.gov.ar
Abstract:The aim of the present study is to evaluate the occurrence of oxidative stress in the cladoceran Daphnia longispina exposed to UV-A and UV-B radiation. The activity of antioxidant enzymes and lipid peroxidation markers is investigated and the protective action of ascorbic acid determined. Results show differences in the lethality radioinduced by UV-A and UV-B. Both UV-A and UV-B exposure cause an important increase in malonaldehyde (MDA) concentration and catalase activity. Ascorbic acid addition reduces the MDA concentration, indicating that the oxidative stress caused by either UV-A or UV-B radiation can be controlled by antioxidants. The increase of the antioxidant enzymes may be a response mechanism to oxidative stress.
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