HPLC and electrophoresis — Competitors or partners |
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Authors: | Lottspeich F |
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Institution: | (1) Genzentrum, Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG |
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Abstract: | Summary Protein structure analysis is an indispensible tool in modern biological research. However, the isolation of a protein or the separation of peptides in a form suitable for sequence analysis is a considerable technical challenge. The two predominant separation methods in protein biochemistry are chromatography and electrophoresis. In this paper the position, advantaged and disadvantages of both HPLC and electrophoresis for the separation of amino acids, peptides and proteins in protein structure analysis are discussed. |
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Keywords: | Column liquid chromatography Electrophoresis Comparison of HPLC and electrophoresis Proteins peptides and amino acids |
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