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Determination of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography coupled to evaporative light scattering detection |
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| Authors: | Toussaint B Duchateau A L van der Wal S Albert A Hubert P Crommen J |
| Institution: | a Department of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, CHU, B 36, B-4000 Liège, Belgium b DSM Research, P.O. Box 18, 6160 MD Geleen, The Netherlands c Department of Biostatistics, University of Liège, CHU, B 23, B-4000 Liège, Belgium |
| Abstract: | A method for the separation and quantitation of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography and evaporative light scattering detection has been developed. Separation of the enantiomers was performed in normal-phase liquid chromatography on a Chiralpak AS chiral stationary phase. The influence of the gas nature, gas pressure and temperature of the drift tube of the evaporative light scattering detector on the detection sensitivity was investigated. The method was validated in terms of linearity, limit of quantitation, accuracy and precision. The enantiomeric excess of (S)-3-tert.-butylamino-1,2-propanediol, used for the industrial synthesis of (S)-timolol, was measured from 0 to 94%. |
| Keywords: | 3-tert -Butylamino-1 2-propanediol |
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