| 血浆和组织中皮质酮的荧光测定法 |
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| 引用本文： | 熊忠,索有瑞.血浆和组织中皮质酮的荧光测定法[J].光谱学与光谱分析,1998,18(2):237-239. |
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| 作者姓名： | 熊忠 索有瑞 |
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| 作者单位： | 中国科学院西北高原生物研究所 |
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| 摘 要： | 皮质酮是肾上腺皮质激素之一。本文试验研究了用荧光分光光度法测定小哺乳动物血浆和组织中皮质酮含量的方法。方法的线性范围为０．０１－０．２４μｇ／ｍＬ，标准回收率平均为９６．０％，相对标准偏差为４．２％。本法具有快速，准确和灵敏度特点，能同时运用于血浆或肾上腺组织中皮质酮的测定。
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| 关 键 词： | 皮质酮 荧光法测定 血浆 组织 测定 |
SPECTROFLUOROMETRIC DETERMINATION OF CORTICOSTERONE IN PLASMA AND TISSUE |
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| Z Xiong,Y Suo.SPECTROFLUOROMETRIC DETERMINATION OF CORTICOSTERONE IN PLASMA AND TISSUE[J].Spectroscopy and Spectral Analysis,1998,18(2):237-239. |
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| Authors: | Z Xiong Y Suo |
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| Institution: | Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001 Xinning. |
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| Abstract: | Corticosterone is one of the adrenal hormones. This paper reported the determination of corticosterone in plasma and tissue of small mammals with spectrofluorometry. The linear relationship between the fluorescence intensity and the concentration of corticosterone is over the range of 0.01-0.24 microg/mL (lambdaex = 470nm, lambdaem = 525nm). The average rate of standard recovery is 96.0% and RSD (n = 10) is 4.2%. This method is simple, rapid and sensitive. |
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| Keywords: | Corticosterone Spectrofluorometric determination |
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