Preparation of immunomagnetic nanoparticles and their application in the separation of mouse CD34 hematopoietic stem cells |
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| Authors: | Xin Liang Jianhe Xu Hebai Shen Jianwen Liu |
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| Institution: | a School of Pharmacy & State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
b Life and Environment Science College, Shanghai Normal University, Shanghai 200234, PR China |
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| Abstract: | The magnetic nanoparticles with a diameter of about 60 nm were synthesized by coprecipitation from ferrous and ferric iron solutions and coated with silica. Then the nanoparticles were modified with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPS) in order to immobilize anti-CD34+ monoclonal antibodies to the surface of modified magnetic particles. The results of transmission electron microscope (TEM) and Fourier transformed infrared (FT-IR) indicated that the nanoparticles were successfully prepared. Scanning electron microscope (SEM) photo confirmed that the mouse CD34+ cells (cells expressing CD34) were separated by the immunomagnetic nanoparticles. The viability of the separated cells was studied by hematopoietic colony-forming assay, the result of which showed that the target cells still had an ability of proliferation and differentiation. The application of the separated CD34+ cells was in testing the pharmacological effect of three samples isolated from enzyme-digested traditional Chinese medicine Colla corii asini. |
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| Keywords: | Nanostructures Magnetic materials Chemical synthesis Magnetic properties |
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