Electron-capture gas chromatographic procedure for simultaneous determination of amphetamine and N-methylamphetamine. |
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| Authors: | P R Paetsch G B Baker L E Caffaro A J Greenshaw G A Rauw R T Coutts |
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| Affiliation: | Department of Psychiatry, University of Alberta, Edmonton, Canada. |
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| Abstract: | An electron-capture gas chromatographic procedure for the simultaneous determination of amphetamine and N-methylamphetamine in biological samples is described. The method employs extraction with the ion-pairing reagent bis(2-ethylhexyl)phosphoric acid, and back-extraction with 0.5 M hydrochloric acid. The hydrochloric acid phase is basified, and the amphetamines and the internal standard benzylamine are derivatized with pentafluorobenzenesulfonyl chloride prior to analysis on a gas chromatograph equipped with a capillary column. Levels of amphetamine and N-methylamphetamine have been determined in the urine and liver of rats treated chronically with (-)-deprenyl. |
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