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Determination and imaging of metabolites from Vitis vinifera leaves by laser desorption/ionisation time‐of‐flight mass spectrometry |
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| Authors: | Gregory Hamm Vincent Carré Anne Poutaraud Benoît Maunit Gilles Frache Didier Merdinoglu Jean‐François Muller |
| Institution: | 1. Laboratoire de Spectrométrie de Masse et de Chimie Laser, Institut Jean Barriol – Fédération de Recherche 2843, Université Paul Verlaine – Metz, 1 Boulevard Arago, 57078 Metz technopole Cedex 03, France;2. INRA, Laboratoire de génétique et d'amélioration de la vigne, UMR1131, 28 rue de Herrlisheim, B.P. 20507, 68021 Colmar Cedex, France |
| Abstract: | Analysis of grapevine phytoalexins at the surface of Vitis vinifera leaves has been achieved by laser desorption/ionisation time‐of‐flight mass spectrometry (LDI‐ToFMS) without matrix deposition. This simple and rapid sampling method was successfully applied to map small organic compounds at the surface of grapevine leaves. It was also demonstrated that the laser wavelength is a highly critical parameter. Both 266 and 337 nm laser wavelengths were used but the 266 nm wavelength gave increased spatial resolution and better sensitivity for the detection of the targeted metabolites (resveratrol and linked stilbene compounds). Mass spectrometry imaging of grapevine Cabernet Sauvignon leaves revealed specific locations with respect to Plasmopara viticola pathogen infection or light illumination. Copyright © 2010 John Wiley & Sons, Ltd. |
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