Synthesis of cationic diacetylene‐co‐carbazole‐co‐fluorene polymers and their sensitive fluorescent quenching properties with DNA |
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| Authors: | Wennan Zeng Shouping Liu Hanxun Zou Linyun Wang Roger Beuerman Derong Cao |
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| Institution: | 1. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China;2. School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Hunan, Xiangtan 411201, China;3. Singapore Eye Research Institute, 11 Third Hospital Avenue, Singapore 168751 |
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| Abstract: | Two neutral precursor conjugated copolymers based 2,7‐diethynylfluorene and 3,6‐diethynylcarbazole units in the main chain ( PFC and PF2C ) were prepared by Hay coupling polymerization. Their cationic copolymers ( CPFC and CPF2C ) were prepared by the methylation of their diethylpropylamino groups with CH3I. For comparison, neutral conjugated homopolymers of 2,7‐diethynylfluorene ( PF ), 3,6‐diethynylcarbazole units ( PC ) and their cationic polymers ( CPF and CPC ) were also prepared with the same method. A comparative study on the optical properties of cationic polymers CPFC and CPF2C in DMF and DMF/H2O showed that they underwent water‐induced aggregation. The spectral behaviors of CPFC and CPF2C with calf thymus DNA showed that a distinct fluorescent quenching took place with minute addition of CT DNA (3.3 × 10?13 M). The results showed that the polymers would be promising biosensor materials for sensitive detection of DNA. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 4168–4177, 2010 |
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| Keywords: | aggregation binding interaction biological applications of polymers cationic conjugated polymers conducting polymers CT DNA fluorescence photophysics UV‐Vis spectroscopy |
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