Liquid chromatography/mass spectrometry analysis revealing preferential occurrence of non‐arachidonate‐containing phosphatidylinositol bisphosphate species in nuclei and changes in their levels during cell cycle |
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| Authors: | Hideo Ogiso Kazuhiro Nakamura Yutaka Yatomi Takao Shimizu Ryo Taguchi |
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| Institution: | 1. Department of Metabolome, Graduate School of Medicine, The University of Tokyo, Bunkyo‐ku, Tokyo 113‐0033, Japan;2. CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332‐0012, Japan;3. Department of Clinical Laboratory, University of Tokyo Hospital, Bunkyo‐ku, Tokyo 113‐8655, Japan;4. Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Bunkyo‐ku, Tokyo 113‐0033, Japan |
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| Abstract: | Phosphatidylinositol phosphates (PtdInsPs) are present within the nucleus, as well as in the membrane. In this mass spectrometry study, different acyl‐containing species of endonuclear PtdInsPs were analyzed in order to clearly understand the role of individual molecular species. A (34:1) acyl‐containing phosphatidylinositol bisphosphate PtdInsP2(34:1)] and PtdInsP2(36:1) were preferentially detected in envelope‐less nuclei prepared from various cultured human cells, while PtdInsP2(38:4) was not a major component within these nuclei. A significant amount of PtdInsP2(34:0) was detected in the HeLa cell nucleus, but not in the A431 and THP‐1 cell nuclei. During the cell cycle in HeLa cells, PtdInsP2(34:0) levels increased in the early G1 phase, and then gradually decreased through S phase, while PtdInsP2(34:1) levels tended to decrease only in late G1 phase and PtdInsP2(38:4) did not change significantly. Thus, individual PtdInsP2 species apparently play different roles in nuclear events based on individual regulation of endonuclear levels. The non‐arachidonate‐containing species were also detected in normal human blood and fluids, suggesting that these minor species may have unique functions in the human body. The techniques used in this study will be applied to clinical studies on a PtdInsPs metabolism. Copyright © 2010 John Wiley & Sons, Ltd. |
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