Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry |
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| Authors: | Xi Shao Xiaoxin Chen Vladimir Badmaev Chi‐Tang Ho Shengmin Sang |
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| Affiliation: | 1. Center for Excellence in Post‐Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA;2. Human Nutrition Research Program, Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University;3. North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA;4. Food Science Graduate Program, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA;5. Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA;6. Cancer Research Program, Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA;7. Laboratory of Applied Pharmacology, 1440‐6 Forest Hill Road, Staten Island, NY 10314, USA |
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| Abstract: | Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono‐demethylated pterostilbene glucuronide, mono‐demethylated pterostilbene sulfate, mono‐hydroxylated pterostilbene, mono‐hydroxylated pterostilbene glucuronide, mono‐hydroxylated pterostilbene sulfate, and mono‐hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MSn (n = 1–3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice. Copyright © 2010 John Wiley & Sons, Ltd. |
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