Screening and analysis of the multiple absorbed bioactive components and metabolites in rat plasma after oral administration of Jitai tablets by high‐performance liquid chromatography/diode‐array detection coupled with electrospray ionization tandem mass spectrometry |
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| Authors: | Shu‐Ping Wang Lei Liu Ling‐Ling Wang Peng Jiang Ji‐Quan Zhang Wei‐Dong Zhang Run‐Hui Liu |
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| Institution: | 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, P.R. China;2. National Engineering Research Center for TCM, Shanghai 201203, P.R. China;3. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, P.R. China |
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| Abstract: | Based on the serum pharmacochemistry technique and high‐performance liquid chromatography/diode‐array detection (HPLC/DAD) coupled with electrospray tandem mass spectrometry (HPLC/ESI‐MS/MS), a method for screening and analysis of the multiple absorbed bioactive components and metabolites of Jitai tablets (JTT) in orally dosed rat plasma was developed. Plasma was treated by methanol precipitation prior to liquid chromatography, and the separation was carried out on a Symmetry C18 column, with a linear gradient (0.1% formic acid/water/acetonitrile). Mass spectra were acquired in negative and positive ion modes, respectively. As a result, 26 bioactive components originated from JTT and 5 metabolites were tentatively identified in orally dosed rat plasma by comparing their retention times and MS spectra with those of authentic standards and literature data. It is concluded that an effective and reliable analytical method was set up for screening the bioactive components of Chinese herbal medicine, which provided a meaningful basis for further pharmacology and active mechanism research of JTT. Copyright © 2010 John Wiley & Sons, Ltd. |
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