A rapid and specific derivatization procedure to identify acyl‐glucuronides by mass spectrometry |
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| Authors: | Alfin D N Vaz Wei Wei Wang Andrew J Bessire Raman Sharma Anne E Hagen |
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| Institution: | Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer Global Research and Development, Groton, CT 06340, USA |
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| Abstract: | A simple procedure is described to identify acyl‐glucuronides by coupled liquid chromatography/mass spectrometry after derivatization to a hydroxamic acid with hydroxylamine. The reaction specificity obviates the need for isolation of the acyl‐glucuronide from an extract. Glucuronides derived from carbamic acids, and alkyl‐ and aromatic amines, are inert to the derivatization reaction conditions, making the hydroxamic acid derivative a fingerprint for acyl‐glucuronides. Copyright © 2010 John Wiley & Sons, Ltd. |
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