| 乙醇-K2HPO4-KH2PO4双水相萃取固体基质室温燐光法测定色氨酸 |
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| 引用本文： | 龙文清,黄春芳,陈华龙.乙醇-K2HPO4-KH2PO4双水相萃取固体基质室温燐光法测定色氨酸[J].分析试验室,2009,28(7). |
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| 作者姓名： | 龙文清 黄春芳 陈华龙 |
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| 作者单位： | 井冈山大学化学化工学院,江西省配位化学重点实验室,吉安,343009 |
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| 摘 要： | 提出了一种基于乙醇-电解质-水体系双水相萃取、固体基质室温燐光法(SS-RTP)测定色氨酸的新方法:将适量电解质和乙醇加入试液中,离心后用SS-RTP测定上相中的色氨酸.在最佳萃取体系乙醇-K2HPO4-KH2PO4-H2O中,测定色氨酸的线性范围1.0×10-7～2.0×10-6mol/L、检出限5.4×10-9mol/L(S/N=3).用于大豆、大米、玉米和竹笋中色氨酸的测定,相对标准偏差2.2%～3.3%,与荧光法比较,相对误差-3.3%～3.2%.
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| 关 键 词： | 双水相萃取 乙醇 固体基质室温燐彝光法 色氨酸 |
Determination of tryptophan by solid surface room temperature phosphorimetry after aqueous two-phase ex- traction based on ethanoI-K2HPO4-KH2PO4-H2O |
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| LONG Wen-qing,HUANG Chun-fang,CHEN Hua-long.Determination of tryptophan by solid surface room temperature phosphorimetry after aqueous two-phase ex- traction based on ethanoI-K2HPO4-KH2PO4-H2O[J].Chinese Journal of Analysis Laboratory,2009,28(7). |
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| Authors: | LONG Wen-qing HUANG Chun-fang CHEN Hua-long |
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| Abstract: | Based on ethanol-electrolyte-H2O system,a new aqueous two-phase extraction was developed for the determination of tryptophan coupled with solid surface room temperature phosphorimetry(SS-RTP).The procedure is as follows: after centrifuged,the sample solution added suitable amount of electrolyte and ethanol was divided into two phases,and then the enriched tryptophan in the upper phase was determined by SS-RTP.In the optimal system of ethanol-K2HPO4-KH2PO4-H2O,the calibration curve of the analyte gave a line... |
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| Keywords: | K2HPO4 KH2PO4 |
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