Regulation of peripheral spindle movement and spindle rotation during mouse oocyte meiosis: new perspectives. |
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| Authors: | Jun-Shu Ai Qiang Wang Shen Yin Li-Hong Shi Bo Xiong Ying-Chun OuYang Yi Hou Da-Yuan Chen Heide Schatten Qing-Yuan Sun |
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| Affiliation: | State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. |
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| Abstract: | Spindle movement, including spindle migration during first meiosis and spindle rotation during second meiosis, is essential for asymmetric divisions in mouse oocytes. Previous studies by others and us have shown that microfilaments are required for both spindle migration and rotation. In the present study, we aimed to further investigate the mechanism controlling spindle movement during mouse oocyte meiosis. By employing drug treatment and immunofluorescence microscopy, we showed that dynamic microtubule assembly was involved in both spindle migration and rotation. Furthermore, we found that the calcium/CaM/CaMKII pathway was important for regulating spindle rotation. |
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