Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents |
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| Authors: | Han Min Su Jung Sang Oh Kim Mahn-Joo Kim Dong H |
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| Affiliation: | Center for Integrated Molecular Systems, and National Research Laboratory of Chirotechnology, Department of Chemistry, Pohang University of Science and Technology, San 31 Hyojadong, Pohang 790-784, Korea. |
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| Abstract: | A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions. |
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