Quantitative determination of chemical constituents from seeds of Nigella sativa L. using HPLC-UV and identification by LC-ESI-TOF |
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Authors: | Avula Bharathi Wang Yan-Hong Ali Zulfiqar Khan Ikhlas A |
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Affiliation: | University of Mississippi, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University, MS 38677, USA. |
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Abstract: | An HPLC method was developed for the simultaneous determination of nine compounds of Nigella sativa L. The separation was achieved within 23 min by using C18 column material, a water-acetonitrile mobile phase, both containing 0.1% acetic acid gradient system and a temperature of 35 degrees C. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of nine compounds were in the range of 0.09-10 and 0.3-25 microg/mL, respectively. The wavelength used for quantification with the diode array detector was 205 and 260 nm. LC/MS coupled with electrospray ionization interface method is described for the identification of compounds in N. sativa L. samples. This method involved the use of [M+H]+ and [M+Na]+ ions in the positive ion mode with extracted ion chromatogram. |
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