An on-line method for determining ATPase bioactivity and its application to human umbilical vein endothelial cell membrane |
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| Authors: | X. F. Yue Y. N. Zhang Z. Q. Zhang |
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| Affiliation: | 1.College of Chemistry and Materials Science,Shaanxi Normal University,Xi’an,China;2.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,Shaanxi Normal University,Xi’an,China;3.Key Laboratory of Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry,Xi’an,China |
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| Abstract: | Based on the determination of inorganic phosphate (the product of ATP dephosphorylation by ATPase), a method for on-line determination of bioactivity of ATPase on cell membrane is presented, in which on-line sampling of cell membrane and separation of inorganic phosphate is employed in a flow injection system. The method has been successfully applied to the investigation of variation of ATPase from human umbilical vein endothelial cell membrane; the results show that ATPase activity decreases with elapsed time studied, which may supply important information for the pertinent studies on cell membrane enzymes bioactivity. |
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