PHOTOREVERSIBLE CHANGES IN pH OF PEA PHYTOCHROME SOLUTIONS |
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| Authors: | Satoru Tokutomi Kotaro T. Yamamoto Yasuhiro Miyoshi Masaki Furuya |
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| Affiliation: | Division of Biological Regulation, National Institute for Basic Biology, Myodaijicho, Okazaki 444, Japan |
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| Abstract: | Abstract— Photoinduced pH changes in unbuffered solutions of undegraded pea phytochrome were studied at 10oC by using a glass electrode. Red light irradiation caused alkalinization of the solutions in the pH range 5.2–xs7.5 and acidification in pH 7.5–8.9. The pH changes were fully reversed by a subsequent irradiation with far-red light. The red and far-red light effects were repeatedly reversible. The solution of tryptic peptide of phytochrome (mol. wt 60000) showed similar photoreversible pH changes. |
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