连续进样的重力驱动微流控芯片流动分析系统

建立了一种可连续进样的集成化重力驱动微流控芯片流动分析系统.该系统可实现连续高通量引入试样,换样时间仅为15s;还采用了水平贮液池和出口引流管等重力驱动流体控制技术,显著降低了试剂和样品的消耗,提高分析速度,增加系统连续工作时间.利用Luminol-K₃[Fe(CN)₆]-H₂O₂化学发光体系考察了该系统的分析性能,系统对不同试样的分析速度达80～100样/h,对H₂O₂检测的线性范围为1×10^-6～1×10^-4mol/L,检出限为2.0×10^-7mol/L,RSD为0.3%(n=5).

A Flow Analysis System Based on Microfluidic Chip with Continuous Sample Introduction and Gravity-driven Flows

An integrated microchip analytical system with gravity-driven flow was developed, equipped a novel continuous sample introduction interface with cannula configuration, featuring high sampling throughput(80-100 samples/h), low sample and reagent consumption and long continuous working time. Stable and fast flow rate for the streams of sample and reagents in microchannel of the chip were achieved by the use of horizontally oriented reservoirs and stretched outlet tubing, respectively.The performance of the system was tested by using H2O2 as a model sample with chemiluminescence detection by the luminol-K3[Fe(CN)6]-H2O2 reaction, achieving a good precision of 0.3%(RSD, n=5), a carryover of 1.1% for sample changing and a detection limit of 2.0×10-7 mol/L.