SPE-GC/MS法检测人血中的鸦片类毒品

采用混合型强阳离子固相萃取柱（SPE）对血液中的吗啡、可待因、6-单乙酰吗啡3种鸦片类毒品进行提取，提取液吹干后，再用N-甲基-双三氟乙酰胺（MBTFA）衍生化，进行GC/MS-SIM检测。以乙基吗啡为内标，3种毒品检测的线性相关系数均大于0.99， 线性范围为10~1000μg/L，相对回收率分别为90%~116 %、90%~110%、79%~102 % ；日内和日间相对标准偏差分别小于10%和16%；检测限分别为1、0.5和3 μg/L。该方法灵敏度高、重现性好、操作简便，可用于鸦片类毒品滥用者或中毒者血液中的毒品及其代谢物的检测。

Analysis of Opiates in Human Blood by Solid-Phase Extraction and Gas Chromatography/ Mass Spectrometry Detection

Morphine, codeine and 6-acetyl morphine in human blood was detected using solid-phase extraction and gas chromatography/mass spectrometry-selected ion monitoring, with ethyl morphine as internal standard, 3 opiates spiked in blank blood were extracted using a solid-phase cartridge. The fluent was evaporated to dryness followed by being derivatized with MBTFA, then it was analyzed directly by GC/MS in selected ion mode. The method was validated in the range 10~1000ng/mL with the drug-spiked blood, the reactor responded linearly over the studied range (R²>0.99 for the 3 drugs); The relative standard deviations (RSD) for intra and inter-days were less than10% and 16%, respectively the relative extraction recoveries vary from 79% to 116%, and LODs were less than 1, 0.5 and 3ng/mL, which promised the perspective application of the method in detecting trace amount of opium in human blood.