| 长白山白眉蝮蛇蛇毒酶的基质辅助激光解吸质谱分析 |
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| 引用本文： | 孙明忠,季怡萍,丁兰,赵大庆,倪嘉缵. 长白山白眉蝮蛇蛇毒酶的基质辅助激光解吸质谱分析[J]. 分析化学, 1999, 27(3): 297-299 |
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| 作者姓名： | 孙明忠 季怡萍 丁兰 赵大庆 倪嘉缵 |
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| 作者单位： | 中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春,130022 |
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| 摘 要： | 用基质辅助激光解吸飞行时间质谱法对长白山眉蝮蛇蛇毒所含４种主要酶：磷脂酶Ａ２，精氨酸酯酶，纤溶酶及Ｌ－氨基酸氧化酶进行了纯度鉴定和分子量测定，结果表明ＭＡＬＤＩ－ＴＯ－ＦＭＸ具有灵敏度高，分辨能力强，分析时间短及样品用量少等优点。用ＭＡＬＤＩ－ＴＯＦＭＳ法分析蛇毒酶的纯度和分子量简捷，快速且重现性好，是ＳＤＳ聚丙烯酰胺凝胶电泳所无法比拟的。
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| 关 键 词： | 基质辅助 激光解吸质谱 白眉蝮蛇 酶 |
The Application of Matrix-assisted Laser Desorption Jonization Mass Spectrometry in Enzymes of Agkistrodon Blomhoffii Ussurensis Venom Analysis |
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| SUN Mingzhong,Ji Yiping,Ding Lan,Zhao Daqing,Ni Jiazuan. The Application of Matrix-assisted Laser Desorption Jonization Mass Spectrometry in Enzymes of Agkistrodon Blomhoffii Ussurensis Venom Analysis[J]. Chinese Journal of Analytical Chemistry, 1999, 27(3): 297-299 |
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| Authors: | SUN Mingzhong Ji Yiping Ding Lan Zhao Daqing Ni Jiazuan |
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| Abstract: | The matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF-MS) spectra of four enzymes (PLA, AEase, Fibrolase, L-a.a. oxidase) in Agkistrodon blomhofEi Ussurensis venom, were given and interpreted. The experiment data showed that MALDI-TOF-MS can be used directly in enzyme analysis with high sensitivity and rapidity. In addition, the results were better than those from sodium dodecyl sulfate-polyacrylamide |
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| Keywords: | Matrix-assisted laser desorption ionization mass spectrometry snake venom enzyme |
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